anti stp1 antibody  (Proteintech)

Journal: Hepatology Communications

Article Title: Dysregulated meta-organismal metabolism of aromatic amino acids in alcohol-associated liver disease

doi: 10.1097/HC9.0000000000000284

Figure Lengend Snippet: Liver expression of enzymes involved in phase II sulfonation and glucuronidation pathways are perturbed in patients with severe alcohol-associated liver disease. Heatmaps were created by log transforming TPM and grouping samples by disease categories, such as HC (n = 10), EAH (n = 12), AHL (n = 18), explant tissue from patients with sAH with liver transplant (ExAH, n = 10), NAFLD (n = 8), HCV (n = 9), and HCV_Cirr (n = 9). (A) Expression of sulfotransferases and phosphosulfate synthase enzymes that comprise the liver sulfonation pathway. (B) Expression of glucuronosyltransferases and phosphoglucomutases involved in the liver glucuronidation pathway. (C) Boxplots of SULT1A1 and SULT2A1 gene expression in TPM of RNA sequencing data from patients with various liver diseases. (D) Boxplots of UGT1A6 and UGT2B4. Error bars indicate SD, and (*) indicates q < 0.05. Abbreviations: AH, alcohol-associated hepatitis; AHL, AH with liver failure; EAH, early alcohol-associated hepatitis; ExAH, explant tissue from patients with severe AH after liver transplant; HC, healthy control; HCV_Cirr, HCV with cirrhosis; sAH, severe alcohol-associated hepatitis; SULT, sulfotransferase; TPM, transcripts per million; UGT, UDP glucuronosyltransferase.

Article Snippet: Proteins for sulfotransferase (SULT) family 1A member 1 (10911-2-AP; Thermo Fisher, Waltham, MA) and UDP glucuronosyltransferase (UGT) family 1 member A6 (PA5-22319; Thermo Fisher, Waltham, MA) were detected after incubation with specific antibodies.

Techniques: Expressing, Gene Expression, RNA Sequencing, Control

Journal: Hepatology Communications

Article Title: Dysregulated meta-organismal metabolism of aromatic amino acids in alcohol-associated liver disease

doi: 10.1097/HC9.0000000000000284

Figure Lengend Snippet: Meta-analysis reveals perturbations of phase II metabolism enzymes in 3 separate bulk RNA sequencing data sets. Violin plots were created by applying a random effects’ model on each of the individual fold changes to create the summarized diamonds, followed by a Bonferroni adjustment for p -values. (A) Log2 fold change, between healthy controls and sAH explant tissue, of sulfotransferases involved in the liver sulfonation pathway. (B) Log2 fold change of phosphoglucomutases involved in the liver glucuronidation pathway. Abbreviations: AH, alcohol-associated hepatitis; AHL, AH with liver failure; EAH, early alcohol-associated hepatitis; HC, healthy control; HCV_Cirr, HCV with cirrhosis; sAH, severe alcohol-associated hepatitis; SULT, sulfotransferase TPM, transcripts per million; UGT, UDP glucuronosyltransferase.

Article Snippet: Proteins for sulfotransferase (SULT) family 1A member 1 (10911-2-AP; Thermo Fisher, Waltham, MA) and UDP glucuronosyltransferase (UGT) family 1 member A6 (PA5-22319; Thermo Fisher, Waltham, MA) were detected after incubation with specific antibodies.

Techniques: RNA Sequencing, Control

Journal: Hepatology Communications

Article Title: Dysregulated meta-organismal metabolism of aromatic amino acids in alcohol-associated liver disease

doi: 10.1097/HC9.0000000000000284

Figure Lengend Snippet: Protein expression of enzymes involved in p-cresol metabolism is decreased in patients with sAH. (A) Liver SULT1A1 and UGT1A6 protein expression measured by western blot from healthy controls and patients with sAH. (B) Densitometric analysis of protein expression for SULT1A1 and UGT1A6 in healthy controls and patients with sAH. Abbreviations: sAH, severe alcohol-associated hepatitis; SULT, sulfotransferase; UGT, UDP glucuronosyltransferase.

Article Snippet: Proteins for sulfotransferase (SULT) family 1A member 1 (10911-2-AP; Thermo Fisher, Waltham, MA) and UDP glucuronosyltransferase (UGT) family 1 member A6 (PA5-22319; Thermo Fisher, Waltham, MA) were detected after incubation with specific antibodies.

Techniques: Expressing, Western Blot

Journal: Journal of Cancer

Article Title: Increased Reactive Oxygen Species and Distinct Oxidative Damage in Resveratrol-suppressed Glioblastoma Cells

doi: 10.7150/jca.45489

Figure Lengend Snippet: Caspase-9 and caspase-3 levels and sulfotransferase 1A1 (SULT1A1) and SULT1C2 expression patterns in untreated and resveratrol-treated U251 and LN428 cells. ( A ) Western blot evaluation of pro-caspase-9, pro-caspase-3, activated caspase-9, and activated caspase-3 U251 and LN428 cells cultured normally (N) and in the presence of 100 µM resveratrol for 48 h (R). ( B ) Fractionation of pro-caspase-9, pro-caspase-3, activated caspase-9, and activated caspase-3 in normally cultured (N) and resveratrol-treated (R) U251 and LN428 cells according to the western blot results. ( C-D ) Western blot and immunocytochemical demonstration of SULT1A1 and SULT1C2 downregulation in U251 cells compared with that in LN428 cells before (N) and after treatment with 100 µM resveratrol for 48 h (R).

Article Snippet: The rabbit anti-human SOD2, Catalase, rabbit SULT1A1 and SULT1C2 (Proteintech, Chicago, IL, USA) were used in the dilution rates of 1:500, 1:500, 1:200, 1:150, respectively.

Techniques: Expressing, Western Blot, Cell Culture, Fractionation

Journal: Journal of Cancer

Article Title: Increased Reactive Oxygen Species and Distinct Oxidative Damage in Resveratrol-suppressed Glioblastoma Cells

doi: 10.7150/jca.45489

Figure Lengend Snippet: Caspase-9 and caspase-3 levels and sulfotransferase 1A1 (SULT1A1) and SULT1C2 expression patterns in untreated and resveratrol-treated U251 and LN428 cells. ( A ) Western blot evaluation of pro-caspase-9, pro-caspase-3, activated caspase-9, and activated caspase-3 U251 and LN428 cells cultured normally (N) and in the presence of 100 µM resveratrol for 48 h (R). ( B ) Fractionation of pro-caspase-9, pro-caspase-3, activated caspase-9, and activated caspase-3 in normally cultured (N) and resveratrol-treated (R) U251 and LN428 cells according to the western blot results. ( C-D ) Western blot and immunocytochemical demonstration of SULT1A1 and SULT1C2 downregulation in U251 cells compared with that in LN428 cells before (N) and after treatment with 100 µM resveratrol for 48 h (R).

Article Snippet: The rabbit anti-human antibodies against SOD2, Catalase, SULT1A1 and SULT1C2 were purchased from Protein Tech (Chicago, IL, USA), and the rabbit anti-human pro-caspase-3, active-caspase-3, pro-caspase-9 and active-caspase-9 antibodies were provided by Abcam (Cambridge, UK).

Techniques: Expressing, Western Blot, Cell Culture, Fractionation